Diacytosis of 125I-asialoorosomucoid by rat hepatocytesa. A non-lysosomal pathway insensitive to inhibition by inhibitors of ligand degradation
Identifieur interne : 003576 ( Main/Exploration ); précédent : 003575; suivant : 003577Diacytosis of 125I-asialoorosomucoid by rat hepatocytesa. A non-lysosomal pathway insensitive to inhibition by inhibitors of ligand degradation
Auteurs : Ta-Min Chang [États-Unis] ; Deborah W. Kullberg [États-Unis]Source :
- BBA - Molecular Cell Research [ 0167-4889 ] ; 1984.
English descriptors
- Teeft :
- Ashwell, Asialoglycoprotein, Asialoglycoprotein receptor, Asialoorosomucoid, Biochem, Biol, Bovine serum albumin, Cell protein, Cell surface ligand, Cells preincubated, Cells preloaded, Chem, Chloroquine, Colchicine, Control dishes, Cytochalasin, Degradation, Degraded, Degraded ligand, Diacytosis, Egta, Expt, Greater extent, Hepatocytes, Inhibitor, Internalization, Intracellular, Leupeptin, Ligand, Ligand degradation, Metal dish, Monensin, Other hand, Perfusion, Perfusion medium, Preincubated, Preloaded, Preloaded cells, Prelysosomal compartment, Receptor, Reincubated, Retention coefficient, Supernatant solution, Tracer, Undegraded, Undegraded ligand, Unlabelled, Unlabelled asialoorosomucoid, Various inhibitors.
Abstract
Abstract: Diacytosis of 125I-asialoorosomucoid by rat hepatocytes was studied by preincubating the cells with the labelled ligand at 37°C for 30 min or 18°C for 2 h, washing free of cell surface receptor-bound tracer at 4°C and then reincubating at 37°C. The cells preloaded at 37°C released a maximum of 18% of the total intracellular ligand as undegraded molecules after 1 h of incubation with an apparent first-order rate constant of 0.018 min−1 (t 1 2 = 39 min). When the preloaded cells were incubated in the presence of 100 μg/ml unlabelled asialoorosomucoid or 5 mM ethylene glycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, the amount of the released ligand increased to 32 and 37%, respectively, without apparent change in kinetics, indicating that these agents prevented rebinding of the released ligand. In the presence of 5 μM colchicine, 20 μM cytochalasin B, 20 μM chloroquine, 10 mM NH4Cl, 10 μM monensin or 20 μM leupeptin, degradation of the preloaded ligand was inhibited, whereas the release of the ligand was either slightly increased or unchanged. Similar effects of leupeptin, colchicine and asialoocrosomucoid were observed with cells preloaded at 18°C. These results indicate that diacytosis of 125I-asialoorosomucoid occurs from a prelysosomal compartment via a route insensitive to inhibition by the inhibitors of ligand degradation.
Url:
DOI: 10.1016/0167-4889(84)90082-X
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C81
- to stream Istex, to step Curation: 000C81
- to stream Istex, to step Checkpoint: 002336
- to stream Main, to step Merge: 003650
- to stream Main, to step Curation: 003576
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Diacytosis of 125I-asialoorosomucoid by rat hepatocytesa. A non-lysosomal pathway insensitive to inhibition by inhibitors of ligand degradation</title><author><name sortKey="Chang, Ta Min" sort="Chang, Ta Min" uniqKey="Chang T" first="Ta-Min" last="Chang">Ta-Min Chang</name></author><author><name sortKey="Kullberg, Deborah W" sort="Kullberg, Deborah W" uniqKey="Kullberg D" first="Deborah W." last="Kullberg">Deborah W. Kullberg</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:058A71D113192688F4AE24FB6E7CAE7484744CE4</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0167-4889(84)90082-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-SKHF5N2W-H/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C81</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C81</idno><idno type="wicri:Area/Istex/Curation">000C81</idno><idno type="wicri:Area/Istex/Checkpoint">002336</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002336</idno><idno type="wicri:doubleKey">0167-4889:1984:Chang T:diacytosis:of:i</idno><idno type="wicri:Area/Main/Merge">003650</idno><idno type="wicri:Area/Main/Curation">003576</idno><idno type="wicri:Area/Main/Exploration">003576</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Diacytosis of 125I-asialoorosomucoid by rat hepatocytesa. A non-lysosomal pathway insensitive to inhibition by inhibitors of ligand degradation</title><author><name sortKey="Chang, Ta Min" sort="Chang, Ta Min" uniqKey="Chang T" first="Ta-Min" last="Chang">Ta-Min Chang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>The Isaac Gordon Center for Digestive Disease and Nutrition, Department of Medicine, The Genesee Hospital, Rochester</wicri:cityArea></affiliation></author><author><name sortKey="Kullberg, Deborah W" sort="Kullberg, Deborah W" uniqKey="Kullberg D" first="Deborah W." last="Kullberg">Deborah W. Kullberg</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>The University of Rochester School of Medicine and Dentistry, Rochester</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">BBA - Molecular Cell Research</title><title level="j" type="abbrev">BBAMCR</title><idno type="ISSN">0167-4889</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">805</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="268">268</biblScope><biblScope unit="page" to="276">276</biblScope></imprint><idno type="ISSN">0167-4889</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4889</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Ashwell</term><term>Asialoglycoprotein</term><term>Asialoglycoprotein receptor</term><term>Asialoorosomucoid</term><term>Biochem</term><term>Biol</term><term>Bovine serum albumin</term><term>Cell protein</term><term>Cell surface ligand</term><term>Cells preincubated</term><term>Cells preloaded</term><term>Chem</term><term>Chloroquine</term><term>Colchicine</term><term>Control dishes</term><term>Cytochalasin</term><term>Degradation</term><term>Degraded</term><term>Degraded ligand</term><term>Diacytosis</term><term>Egta</term><term>Expt</term><term>Greater extent</term><term>Hepatocytes</term><term>Inhibitor</term><term>Internalization</term><term>Intracellular</term><term>Leupeptin</term><term>Ligand</term><term>Ligand degradation</term><term>Metal dish</term><term>Monensin</term><term>Other hand</term><term>Perfusion</term><term>Perfusion medium</term><term>Preincubated</term><term>Preloaded</term><term>Preloaded cells</term><term>Prelysosomal compartment</term><term>Receptor</term><term>Reincubated</term><term>Retention coefficient</term><term>Supernatant solution</term><term>Tracer</term><term>Undegraded</term><term>Undegraded ligand</term><term>Unlabelled</term><term>Unlabelled asialoorosomucoid</term><term>Various inhibitors</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Diacytosis of 125I-asialoorosomucoid by rat hepatocytes was studied by preincubating the cells with the labelled ligand at 37°C for 30 min or 18°C for 2 h, washing free of cell surface receptor-bound tracer at 4°C and then reincubating at 37°C. The cells preloaded at 37°C released a maximum of 18% of the total intracellular ligand as undegraded molecules after 1 h of incubation with an apparent first-order rate constant of 0.018 min−1 (t 1 2 = 39 min). When the preloaded cells were incubated in the presence of 100 μg/ml unlabelled asialoorosomucoid or 5 mM ethylene glycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, the amount of the released ligand increased to 32 and 37%, respectively, without apparent change in kinetics, indicating that these agents prevented rebinding of the released ligand. In the presence of 5 μM colchicine, 20 μM cytochalasin B, 20 μM chloroquine, 10 mM NH4Cl, 10 μM monensin or 20 μM leupeptin, degradation of the preloaded ligand was inhibited, whereas the release of the ligand was either slightly increased or unchanged. Similar effects of leupeptin, colchicine and asialoocrosomucoid were observed with cells preloaded at 18°C. These results indicate that diacytosis of 125I-asialoorosomucoid occurs from a prelysosomal compartment via a route insensitive to inhibition by the inhibitors of ligand degradation.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Chang, Ta Min" sort="Chang, Ta Min" uniqKey="Chang T" first="Ta-Min" last="Chang">Ta-Min Chang</name></region><name sortKey="Kullberg, Deborah W" sort="Kullberg, Deborah W" uniqKey="Kullberg D" first="Deborah W." last="Kullberg">Deborah W. Kullberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003576 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003576 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:058A71D113192688F4AE24FB6E7CAE7484744CE4
|texte= Diacytosis of 125I-asialoorosomucoid by rat hepatocytesa. A non-lysosomal pathway insensitive to inhibition by inhibitors of ligand degradation
}}
| This area was generated with Dilib version V0.6.33. |  |